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Santa Cruz Biotechnology
glucose 6 phosphatase g6pase  Glucose 6 Phosphatase G6pase, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/product/g6pase+%CE%B2/G6Pase-%CE%B2+Lentiviral+Activation+Particles/pmc05495913-13-0-3 Average 93 stars, based on 1 article reviews
glucose 6 phosphatase g6pase - by Bioz Stars,
2026-10
93/100 stars
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Santa Cruz Biotechnology
g6pase β  G6pase β, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/product/g6pase+%CE%B2/G6Pase-%CE%B2+(m)-PR/pmc04211855-51-41-44 Average 93 stars, based on 1 article reviews
g6pase β - by Bioz Stars,
2026-10
93/100 stars
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CRISPR/Cas9 KO Plasmids consists of G6Pase-β-specific 20 nt guide RNA sequences derived from the GeCKO (v2) library. For CRISPR gene knockout, gRNA sequences direct the Cas9 protein to induce a site-specific double strand break (DSB)
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Buy from Supplier |
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CRISPR/Cas9 KO Plasmids consists of G6Pase-β-specific 20 nt guide RNA sequences derived from the GeCKO (v2) library. For CRISPR gene knockout, gRNA sequences direct the Cas9 protein to induce a site-specific double strand break (DSB)
|
Buy from Supplier |
|
Gene Silencers generally consist of pools of three to five target-specific 19-25 nucleotide sequences in length. For independent verification of G6Pase-β gene silencing results, individual duplex components or plasmids are also available upon request.
|
Buy from Supplier |
|
Gene Silencers generally consist of pools of three to five target-specific 19-25 nucleotide sequences in length. For independent verification of G6Pase-β gene silencing results, individual duplex components or plasmids are also available upon request.
|
Buy from Supplier |
|
Gene Silencers generally consist of pools of three to five target-specific 19-25 nucleotide sequences in length. For independent verification of G6Pase-β gene silencing results, individual duplex components or plasmids are also available upon request.
|
Buy from Supplier |
|
CRISPR/Cas9 KO Plasmids consists of G6Pase-β-specific 20 nt guide RNA sequences derived from the GeCKO (v2) library. For CRISPR gene knockout, gRNA sequences direct the Cas9 protein to induce a site-specific double strand break (DSB)
|
Buy from Supplier |
|
Gene Silencers generally consist of pools of three to five target-specific 19-25 nucleotide sequences in length. For independent verification of G6Pase-β gene silencing results, individual duplex components or plasmids are also available upon request.
|
Buy from Supplier |
|
Gene Silencers generally consist of pools of three to five target-specific 19-25 nucleotide sequences in length. For independent verification of G6Pase-β gene silencing results, individual duplex components or plasmids are also available upon request.
|
Buy from Supplier |
|
CRISPR/Cas9 KO Plasmids consists of G6Pase-β-specific 20 nt guide RNA sequences derived from the GeCKO (v2) library. For CRISPR gene knockout, gRNA sequences direct the Cas9 protein to induce a site-specific double strand break (DSB)
|
Buy from Supplier |
Image Search Results
Journal: American Journal of Physiology - Gastrointestinal and Liver Physiology
Article Title: Nutrient sensing by absorptive and secretory progenies of small intestinal stem cells
doi: 10.1152/ajpgi.00416.2016
Figure Lengend Snippet: Primer sequences of target genes
Article Snippet:
Techniques:
Journal: American Journal of Physiology - Gastrointestinal and Liver Physiology
Article Title: Nutrient sensing by absorptive and secretory progenies of small intestinal stem cells
doi: 10.1152/ajpgi.00416.2016
Figure Lengend Snippet: Primary antibodies used for immunofluorescence
Article Snippet:
Techniques:
Journal: American Journal of Physiology - Gastrointestinal and Liver Physiology
Article Title: Nutrient sensing by absorptive and secretory progenies of small intestinal stem cells
doi: 10.1152/ajpgi.00416.2016
Figure Lengend Snippet: Immunofluorescence staining of proliferation and differentiation biomarkers in typical (A), ISC (B), enterocyte (C), and goblet (D) organoids. Nuclei are stained blue. Organoids were stained with stem cell marker OLFM4 (red), Paneth cell marker lysozyme (LYZ; green), enterocyte marker sucrase isomaltase (SI; red), goblet cell marker mucin 2 (MUC2; green), or enteroendocrine marker chromogranin-A (CHGA; green). Representative typical, ISC, enterocyte, and goblet organoids are shown at ×60 magnification. Bars = 25 μm. Occasional basolateral staining has been shown (personal observations) to be caused largely by autofluorescence of the Matrigel. Frequency of marked cells from at least 5 organoids from 3 mice (vi of each organoid type, a,b,c,dP ≤ 0.05). Immunocytochemistry of the fructose-responsive enzyme glucose-6-phosphatase (G6Pase) in enterocyte, ISC, and secretory goblet organoids is shown (E). All organoid types were analyzed on the 7th day of experiment, which for goblet, Paneth, and enterocyte organoids, is the 4th day after differentiation (see Fig. 1). The control panel is an image of an enterocyte organoid incubated without the primary antibody.
Article Snippet:
Techniques: Immunofluorescence, Staining, Marker, Immunocytochemistry, Control, Incubation
Journal: American Journal of Physiology - Gastrointestinal and Liver Physiology
Article Title: Nutrient sensing by absorptive and secretory progenies of small intestinal stem cells
doi: 10.1152/ajpgi.00416.2016
Figure Lengend Snippet: Effect of cell type on the mRNA expression of classical fructose-responsive genes Glut5, G6Pase, and Khk. Typical and ISC organoids were incubated in their respective culture media for 6 days after generation from crypt precursors. Enterocyte and goblet organoids were incubated in their respective culture media for 3 days after directed differentiation from ISC precursors. Stem cells with very few Paneth cells constituted ISC organoids; enterocytes made up almost entirely enterocyte organoids, with few goblet and occasional enteroendocrine cells, whereas goblet organoids consisted primarily of goblet with some enteroendocrine cells. Media in each organoid type was supplemented with an additional 5 mM glucose, 5 mM fructose, or 10 mM fructose for 24 h. Levels of mRNA in all organoids were normalized to that in typical organoids exposed to 5 mM glucose (1.0). Only typical, enterocyte, and goblet organoids responded to supplemental fructose. Expression of the control gene Sglt1 did not vary with fructose. These results were confirmed 3 other times, using ISC, enterocyte, and goblet organoids from different mice. Analysis was by 2-way ANOVA, a,b,cP ≤ 0.05 within cell type, n = 3.
Article Snippet:
Techniques: Expressing, Incubation, Control
Journal: American Journal of Physiology - Gastrointestinal and Liver Physiology
Article Title: Nutrient sensing by absorptive and secretory progenies of small intestinal stem cells
doi: 10.1152/ajpgi.00416.2016
Figure Lengend Snippet: Effect of fructose incubation of organoids enriched in Paneth cells on fructose-responsive genes Glut5, G6Pase, and Khk. Because Paneth cells also arise from secretory progenitors, we made (i to iv) then challenged Paneth cell-enriched organoids with 10 mM fructose (10 mM glucose as control, v). *P < 0.05, n = 3; for i-iii, a,b,c,dP < 0.05). Fructose induced marked increases in expression of Glut5, G6Pase, Khk, and Si genes. vi: Challenging ISC-enriched organoids with 20 mM fructose to increase the concentration gradient still had no effect on glucose-responsive genes.
Article Snippet:
Techniques: Incubation, Control, Expressing, Concentration Assay
Journal: American Journal of Physiology - Gastrointestinal and Liver Physiology
Article Title: Nutrient sensing by absorptive and secretory progenies of small intestinal stem cells
doi: 10.1152/ajpgi.00416.2016
Figure Lengend Snippet: Effect of glucose concentration in the incubation medium on fructose-responsive genes. The media in typical and enterocyte organoids was changed from 17.5 to 11.5 mM glucose (control: 17.5 to 17.5 mM) at the same time that fructose was added 24 h before the termination of the experiment. We added 5 mM glucose or 5 mM or 10 mM fructose to organoids in normal 17.5 mM or low 11.5 mM glucose media (results separated by a dashed line). Although there was an expected effect of fructose on expression of Glut5 (i) and G6Pase (ii), there was no effect of glucose concentration in the media. Alpi (iii) expression was independent of media and sugar in both typical and enterocyte organoids. There was also an effect of cell type on the expression of these genes that are highly expressed in enterocytes in vivo. Expression was normalized to that in 5 mM glucose of the control 17.5 to 17.5 mM experiment (1.0). Analysis was by 3-way ANOVA (see text for details), n = 3.
Article Snippet:
Techniques: Concentration Assay, Incubation, Control, Expressing, In Vivo
Journal: American Journal of Physiology - Gastrointestinal and Liver Physiology
Article Title: Nutrient sensing by absorptive and secretory progenies of small intestinal stem cells
doi: 10.1152/ajpgi.00416.2016
Figure Lengend Snippet: Effect of Glut5 and Khk deletion on fructose sensing by typical organoids. Typical organoids from wild-type (WT), Glut5−/−, and Khk−/− mice were cultured for 6 days then incubated in media supplemented with 5 mM glucose, 5 mM fructose, or 10 mM fructose for 24 h on the final day of culture. There was no effect of genotype on expression of undeleted genes. Levels of mRNA in 5 and 10 mM fructose were normalized to that in 5 mM glucose (1.0) for each genotype. Except for G6Pase expression, which did not require glucose transporter 5 (GLUT5), fructose transport and metabolism were clearly necessary for fructose sensing. a,b,cP ≤ 0.05 within genotype, n = 3.
Article Snippet:
Techniques: Cell Culture, Incubation, Expressing
Journal: American Journal of Physiology - Gastrointestinal and Liver Physiology
Article Title: Nutrient sensing by absorptive and secretory progenies of small intestinal stem cells
doi: 10.1152/ajpgi.00416.2016
Figure Lengend Snippet: Effect of CHIR-induced dedifferentiation of enterocyte organoids on expression of genes involved in the fructose response and in marking cell types. From ISC precursors, enterocyte organoids were cultured for 3 days with C59 +VPA to full differentiation as indicated by peak expression of fructose-responsive genes. To force dedifferentiation, the Wnt inhibitor C59 was removed from the medium, and enterocyte organoids were then exposed for 36 h to 3 (dENT1) or 6 μM CHIR (dENT2) or for 6 days to 6 μM CHIR (dENT3). ENT, dENT1, dENT2, dENT3, and ISC organoids from the same mouse were then challenged with 5 mM fructose in the last 12 h before harvest. Gene expression was normalized to glucose-incubated, enterocyte organoids. Although expression of fructose-responsive genes decreased markedly with CHIR treatment, Glut5 and G6Pase expression still responded to fructose incubation in all dedifferentiated enterocyte organoids. Lgr5+ levels increased almost 100-fold with CHIR-induced dedifferentiation but never reached levels observed in ISC organoids. Lyz and Muc2 expression were also affected by dedifferentiation (n = 3 per treatment).
Article Snippet:
Techniques: Expressing, Cell Culture, Gene Expression, Incubation
Journal: Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy
Article Title: Expression of gluconeogenic enzymes and 11β-hydroxysteroid dehydrogenase type 1 in liver of diabetic mice after acute exercise
doi: 10.2147/DMSO.S70767
Figure Lengend Snippet: Hepatic protein expression of glucose-6-phosphatase α (G6Pase α) measured 8 hours after acute exercise in db/db mice. Notes: Values are reported as a mean ± standard error of the mean for 6–9 mice in each group, and normalized to internal control.
Article Snippet: Blots were incubated with primary antibody to GR (1:1000; Cell Signaling Technology, Danvers, MA, USA), PEPCK (1:200; Santa Cruz Biotechnology Inc., Dallas, TX, USA), 11β-HSD1 (1:1000; Abcam Cambridge, MA, USA), G6Pase α (1:200; Santa Cruz Biotechnology Inc., Dallas, TX, USA), and
Techniques: Expressing, Control
Journal: Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy
Article Title: Expression of gluconeogenic enzymes and 11β-hydroxysteroid dehydrogenase type 1 in liver of diabetic mice after acute exercise
doi: 10.2147/DMSO.S70767
Figure Lengend Snippet: Hepatic protein expression of glucose-6-phosphatase β (G6Pase β) measured 8 hours after acute exercise in db/db mice. Notes: Values are reported as a mean ± standard error of the mean for 6–9 mice in each group, and normalized to internal control.
Article Snippet: Blots were incubated with primary antibody to GR (1:1000; Cell Signaling Technology, Danvers, MA, USA), PEPCK (1:200; Santa Cruz Biotechnology Inc., Dallas, TX, USA), 11β-HSD1 (1:1000; Abcam Cambridge, MA, USA), G6Pase α (1:200; Santa Cruz Biotechnology Inc., Dallas, TX, USA), and
Techniques: Expressing, Control